Study of oxidative stress and antioxidant status in ascitic patients with ovarian cancer in comparison to liver cirrhosis patients
- MOJ Proteomics & Bioinformatics
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Khaleel Pasha,1 Gopal Reddy M,2 Ramesh Kumar B,1 Qamar Ayesha,1 Srinivasulu M,3 Suseela K,4 Gita Sharma,5 Livy Alex6
Department of Gastroenterology, Osmania General Hospital, India - Khaleel Pasha, Department of Gastroenterology, Osmania General Hospital, India
- Gopal Reddy M, Department of Biochemistry, Malla Reddy Narayana Multispeciality Hospital Suraram, India
- Ramesh Kumar B, Department of Gastroenterology, Osmania General Hospital, India
- Qamar Ayesha, Department of Gastroenterology, Osmania General Hospital, India
- Srinivasulu M, Department of Surgical Oncology, MNJ Institute of Oncology and Regional Centre, India
- Suseela K, Department of Pathology, Basavatarakam Indo-American Cancer Hospital & Research Institute, India
- Gita Sharma, Tapadia Diagnostics Centre, India
Abstract
Abstract
Background: The pathological accumulation of abdominal fluid (ascites) is due to multiple causes often associated either with peritoneal diseases or non-peritoneal diseases. Oxidative stress is caused by free radicals leading to oxidative destruction of polyunsaturated fatty acids constitutive of cellular membrane. The present study was conducted to assess the oxidative stress and antioxidant status in ascitic patients with liver cirrhosis in comparison to patients with ovarian carcinoma. Serum and ascitic fluid concentrations of malondialdehyde (MDA), Superoxide dismutase(SOD/gm.protein) and Ceruloplasmin (CP) was assessed in 375 ascitic patients, among them 145 patients were females with ovarian cancer (Age 19-84 yrs; mean age 49.85±11.75); 230 were liver cirrhosis patients (178 males and 52 females age 20-75 yrs; mean age 46.59±10.82) and 150 subjects were healthy controls (91 males and 59 females age 24-65 yrs; mean age 43.31±8.51). MDA and SOD wasestimated by manual methods; Ceruloplasmin(CP) was measured by immunoturbidometric assay using commercial kit. Significant increased levels of serum and ascitic fluid MDA and CP and decreased levels of SOD was observed in ovarian cancer patients when compared to that of liver cirrhosis and control group (p<0.001). Significant increased levels of serum MDA and decreased levels of CP and SOD was observed in liver cirrhosis patients when compared to that of control (p<0.001).
Conclusion:It could be inferred from the experiments conducted that MDA and CP increased in ovarian cancer patients while there was a reduction in SOD. MDA showed the same trends in liver cirhossis patients however CP and SOD showed decreased levels in liver cirrhosis patients. Thus it is possible that CP could seem as a biomarker for screening of ovarian carcinoma. The financial implication for screening also would be affordable to the common man. Thus the most salient feature of this communication is identification of a suitable biomarker for screening ovarian cancer which is affordable. CP levels thus could prove a valuable assay for the screening, diagnosis and monitoring of ovarian cancer.
Keywords
ascites, liver cirrhosis, ovarian cancer, oxidative stress, ceruloplasmin, biomarker, antioxidants
