The role of two parasitological staining techniques in diagnosis of cryptosporidiosis among diarrhoeic patient’s admitted to kosti teaching hospital, white nile state, sudan
- MOJ Public Health
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Abdelhakam G Tamomh,1,2 Mohammed A Suliman,2 Kabulo K Cedric,3 Hafiz Y Mohammed,2 Abdalmoneim M Magboul,2 Ibrahim M Hassan,2 Rabah M Ibrahim,2 Ammar A Abdalla,2 Tarteel A Abdallah,2 Ahmed A Alfaki2
College of Medical laboratory sciences, Dalian Medical University, China; Department of Stomatology, Dalian Medical University, China
Abstract
Background: Cryptosporidiosis is the disease caused byCryptosporidium parvum, whichassociated with economic losses and the public health problems in human.
Objectives: The aim of this study was to detectC.parvum, compare between modified ZNstaining technique and trichrome staining and study the role of a parasitological techniques for recovery of cryptosporidiosis among diarrheic patient’s admitted to Kosti teaching hospital, White Nile State, Kosti city, Sudan. This representative is a comparative study was conducted from August 2016 to April 2017.
Methods: A total of three hundred stool samples were collected from diarrheic patients, thesamples were examined using Formal ether concentration technique and staining techniques and the samples were preserved in schauddins fixative.
Results: The overall results of parasite detected by modified ZN stain was 18%, and bytrichrome stain 5%. The detection of C.parvum was higher in female than male.
Conclusion: The study showed that modified ZN staining technique is the most sensitiveand accurate so will be recommended to be use as first choice in diagnosis of Cryptosporiduim parvum.
Keywords
Cryptosporidiosis, modified ZN staining technique, Trichrome staining technique, parasitological techniques, dairy farmers, population, diahrriac patient, parasite, health facilities, stool
