Abstract

Phytochemical screening and antibacterial activities of Dichloromethane leaves extract of Leptadenia hastata was investigated to evaluate the active component of the plant. Phytochemical analysis of the aerial parts showed the presence of bioactive secondary metabolites; alkaloids, steroids, terpenoids, saponins, flavonoids, Carbonhydrate, Glycoside are present while tannins, and saponin were absent. The in vitro antimicrobial activity against four bacterial strains Escherichia coli, Salmonella typhii, Staphylococcus aureus and Klebsielia pneumonia, was also assessed. The results; Alkaloids, steroids, terpenoids, saponins, flavonoids, Carbohydrate, Glycoside were the main compounds of the Dichloromethane extract from Leptadenia hastata. This extract has significant activity, they exhibited an antimicrobial property against bacterial strains. Escherichia coli and Staphylococcus aureus were the most sensitive strains with inhibitory concentration values of 1.23 ± 0.06mm and 1.33 ± 0.06mm at 1000ppm respectively. In conclusion; Leptadenia hastata Dichloromethane Leaves extract was found to be selectively antimicrobial as a result of the pharmacologically active components, which support its Ethno-botanical claims of traditional Medical practitioners in the treatment of various ailments.

Keywords

dichloromethane, phytochemical, Leptadenia, hastata, metabolites, antibacterial, screening, pathogens, Escherichia, coli, Salmonella, typhii, Staphylococcus, aureus, Klebsielia, pneumonia