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Evaluation of the cardioprotective role of 7-hydroxy-4-methylcoumarin on isoproterenol induced myocardial infarction in rats


MOJ Drug Design Development & Therapy
Eakta Sharma, Ajay Singh Kushwah, Taranbir Singh
Department of Pharmacology, Amar Shaheed Baba Ajit Singh Jujhar Singh Memorial College of Pharmacy, India

Abstract

Coumarins are the oldest class naturally occurring benzopyrene derivatives, associated with remarkable pharmacological activities, one of the most potent 7-hydroxy-4-methylcoumarin (7H4MC) shown the presence of various pharmacological activities. Thus, the present study aims to evaluate the cardioprotective effect of 7-hydroxy-4-methylcoumarin on isoproterenol (ISO) induced myocardial infarction in rats, experimental model. Myocardial infarction was induced by subcutaneous injection of ISO at the dose of (5.25 and 8.5mg kg-1) on two consecutive days (29th day and 30th day), 7H4MC at two doses (100 and 200 mg kg-1) was administered orally for 30days before Iso administration. Wistar rats were randomly assigned in experimental groups animals serve as a saline control, saline plus ISO-treated, standard drug (carvedilol 1mg kg-1 p.o.) plus ISO treatments, test drug only treated groups received 7H4MC (100 and 200mg kg-1 p.o) and test groups treated with the 7H4MC low dose and higher dose with ISO. The ISO-treated rats show a significant elevation in cardiac toxicity markers enzymes, lipid peroxidation; modulate oxidative enzyme level, infarct size, and hemodynamic parameters. The deformed by ISO was restored significantly by oral administration of 7H4MC. Test drug dose 200mg kg-1 is more effective and it was also shown nearly similar results as that of standard drug. Our study demonstrates that 7H4MC possessing antioxidant activity and has a significant protective effect against ISO-induced myocardial infarction. We believe that pre-treatment with 7H4MC may contribute to developing novel strategies in the prevention and treatment of cardiotoxic effects of elevated level of catecholamines.

Keywords

Isoproterenol, 7-Hydroxy-4-methylcoumarin, Carvedilol, Antioxidant activity, Cardiac marker enzymes, Catecholamines.

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