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Klotho and catalase expression in essential hypertension


Background: Klotho, an anti-aging gene, has emerged as novel inhibitor of oxidative stress at cellular level. Forkhead box-O (FOXO) proteins regulate crucial cellular processes, including stress-resistance, metabolism, cell-cycle arrest, and apoptosis. Klotho regulates FOXOs via inhibition of insulin/IGF-1 pathway. In rat glomerular-mesangial cells, FOXO1 has been shown to regulate Catalase expression.
Aim: This study aimed at determining Klotho and Catalase gene expressions and Catalase activity in peripheral blood mononuclear cells (PBMCs) of Indian essential hypertensive patients as compared to normotensive healthy controls.
Methods: Forty-eight hypertensives and 48 age, BMI-matched controls were recruited. Gene expression was evaluated by quantitative Real-Time PCR. Catalase enzyme activity in PBMCs and serum soluble α-Klotho levels were detected using Enzyme-Linked Immunosorbent Assay.
Results: Gene expressions for Klotho (p<0.001) and FOXO1 (p=0.002) were significantly low in patients as compared to controls. Catalase expression was also low but did not reach statistical significance. However, there was strong positive correlation between ΔCt based gene expression of Klotho and Catalase in patients (p<0.001) as well as controls (p=0.008). Positive correlation was also observed between gene expression of FOXO1 and that of Klotho (p=0.006) and catalase (p=0.001) in hypertensives. Catalase activity in patients were significantly low (p<0.001) as compared to controls and significantly correlated with soluble α-Klotho levels (rs=0.32, p=0.027) which were also reduced by 30.2% (p<0.001) in patient group.
Conclusion: Present study demonstrates low soluble levels and gene expression of anti-aging protein Klotho in hypertensives. Klotho may influence Catalase expression in essential hypertension through its effect on FOXO1 expression.


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