Abstract

Cordyceps militaris is a valuable medicinal mushroom. In this study, nutritional compositions and fermentation conditions, including pH and ratio of seeding stock, were investigated to evaluate  the  growth  of  this  mushroom  in  liquid  culture.  Among  the  investigated  carbon sources, glucose showed highest efficiency with colony density reaches 16.8 colonies per ml, colony  diameter  reaches  1.4mm,  and  mycelial  biomass  reaches  12.42g/l.  The  most  suitable  source  of  nitrogen  is  silkworm  pupae  extract  with  the  colony  density  reaches19.2colonies/ml, colony diameter reaches 1.6mm, and mycelial biomass reaches 15.53g/l. The most suitable sources of mineral salts are K₂HPO₄ and MgSO₄.7H₂O. The pH of the culture  medium  was  determined  at  6.  The  appropriate  ratio  of  seeding  stock  for  biomass  production was determined at 5.108/ml.

Keywords

nutritional compositions, Cordyceps militaris, colony density, submerged culture, cancer cells, extract, sodium nitrate