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Alteration in the activities of antioxidant enzymes and lipid peroxidation in mice transplanted with Dalton’s lymphoma ascites tumor by Helicia Nilagirica Bedd


MOJ Anatomy & Physiology
Ganesh Chandra Jagetia, Jennifer Zoremsiami

Abstract

The present study was performed to obtain an insight into the biochemical profile of Dalton’s lymphoma ascites tumour in Swiss albino mice treated with aqueous extract of Helicia nilagirica. The mice bearing Dalton’s lymphoma ascites tumor was injected with 175 mg/kg body weight of aqueous extract of H. nilagirica for nine consecutive days. Thereafter, the tumour cells were aspirated at 2, 4, 6, 8, 12 and 24h post drug treatment for the estimation of glutathione, glutathione-s-transferase, catalase, superoxide dismutase, and lipid peroxidation. The administration of tumorized mice with H. nilagirica aqueous extract caused a significant depletion in the glutathione contents and activities of glutathione-s-transferase, catalase, superoxide dismutase in a time dependent manner up to 24 h post assay time, when compared to sterile physiological saline treated group. In contrast, treatment of tumorized mice with H. nilagirica aqueous extract resulted in a time dependent rise in the lipid peroxidation when compared to sterile physiological saline treated group. Our study demonstrates that the cytotoxic effect of H. nilagirica aqueous extract on Dalton’s lymphoma ascites tumour cells may be due to increased lipid peroxidation and reduction in the glutathione contents, activities of glutathione-s-transferase, catalase, and superoxide dismutase.

Keywords

mice, helicia nilagirica, dalton’s lymphoma, glutathione, antioxidant enzymes, lipid peroxidation, nicotinamide, natural products, new drug discovery, urinary tract infections, lipid peroxidation, food pellets, non-infected stem bark, sterile physiological saline, tumorized animals

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