Bio isolation, chemical purification, identification, antimicrobial and synergistic efficacy of extracted essential oils from stem bark extract of Spondias mombin(Linn)
- International Journal of Molecular Biology: Open Access
Oludare Temitope Osuntokun,1 Gamberini M Cristina2
The purpose of this research work is to isolate, purify, chemical identification, antimicrobial and Synegistic efficacy of extracted essential oils from ethyl acetate extract of Spondias mombin. The ethyl acetate stem bark extract of Spondias mombin were air-dried, chopped into smaller pieces and cold extracted exhaustively with ethylacetate. The crude extract was partitioned using various solvents and the dichloromethane fraction was concentrated and fractionated using column chromatography packed with Si-gel and Sephadex-LH and eluted with appropriate solvent systems accordingly. In order to obtain pure extracts, partially purified fractions were further purified. The structures of the isolated compounds were determined by using data obtained from GC-MS spectrum. The compound isolated includeAspidofractinine-3-methanol, Phthalic acid, 2-ethylhexyl tetradecyl ester, Phthalic acid, di (2-propylpentyl) ester), (9-(2', 2’-Dimethylpropanoilhydrazono)-3,6-dichloro-2,7-bis-[2-(diethylamino)-ethoxy]fluorine) and Tere phthalic acid, dodecyl 2-ethylhexyl ester.These compounds have individual and synergistic activity against Gram negative (E.coli), Gram-positive (Bacillus subtilis) and (Aspergillusflavus) at 10, 5, 2.5 and 1.25g/mL.
spondias mombin, antimicrobial, essential oils, gc-ms