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Antithrombotic and anti-inflammatory activities of leaf methanolic extract of Euphorbia hirta Lin


International Journal of Complementary & Alternative Medicine
Mushiur Rahman SM,1 Sohel Rana,1 Ariful Islam AFM,2 Alok Kumer,3 Abu Kayes Hashem,4 Habibur Rahman,1 Mohaimenul,1 Tonmoy Kumar Biswas,3 Sayeed Sabbir AA1

Abstract

Euphorbia hirta (Euphorbiaceae family) has been claimed as a useful folk medicine to traditional healers for the treatment of fever, pain, diarrhoea, peptic ulcers, vomiting, asthma, bronchitis, inflammation, kidney stones, and menstrual problems and as anti-venom. Therefore, the intention of our study was to evaluate the possible antithrombotic and anti-inflammatory activities of methanolic extract of Euphorbia hirta leaves in mice. The in-vitro clot lysis test was performed to investigate the antithrombotic activity of Euphorbia hirta leaf methanolic extract. In addition, xylene-induced ear edema and cotton pellet-induced granuloma formation tests were conducted to evaluate the anti-inflammatory effect of methanolic extract of Euphorbia hirta leaves in mice. Crude methanolic extract was administrated orally at a dose of 200, 400 and 800mg/kg in mice. Treatment with crude extract of Euphorbia hirta leaves (100 and 200mg/mL) restrained the in-vitro thrombosis process. In this test, MEHL 200mg/mL showed the strongest inhibition value (38.00±2.52 %) of thrombosis. The treatment with MEHL at a dose of 200, 400 and 800 mg/kg showed a dose-dependent significant inhibition of ear edema in mice, whereas the highest inhibition value was exhibited by MEHL 800mg/kg (58.78±2.90%). Moreover, all of the dosages (200, 400 and 800mg/kg) of MEHL were significantly reduced the granuloma formation in mice as well as decrease the body weight gain. Here, MEHL 800mg/kg provided highest inhibition value (56.80±3.85 %) of granuloma formation in mice. Furthermore, no sign of toxicity, behavioral changes or mortality was observed upto the dose of 3200 mg/kg for MEHL or control group. The present investigation explores that methanolic extract of Euphorbia hirta leaves has moderate antithrombotic and significant anti-inflammatory effects. These outcomes strongly support the traditional uses of this plant scientifically, in case of thrombosis and inflammation management.

Keywords

Euphorbia hirta, thrombolysis, anti-inflammatory, cotton pellet, aggregation, blood vessel injury, endogenous, ischemic myocardial, atherosclerotic plaques, antithrombotic, armamentarium, aspirin, streptokinase, warfarin, nonsteroidal, corticosteroids, arachidonic

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