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Experimental model in rats of penumbra area using implemented baloon in caudate nucleus volume dependent and time dependent

Journal of Neurology & Stroke
João Pedro Franco Leal de Oliveira,1 Túlio Melo Oliveira,1 Vitor Pelogi Arienzo,1 Henrique Álvares Pessoa,1 Valquíria Neves Perin,1 Paulo Henrique Pires de Aguiar2


The study aims to determine whether early removal of an experimental intracerebral mass alters neurological function. In four experimental series, 0.6mL and 0.8mL balloons were implanted by stereotaxis and inflated into the right caudate nucleus of rats. After 1hour of insufflation, the brains were removed and studied by histopathological analysis. Immunohistochemical was included with protein S-100, marker of neuronal destruction. Four groups were formed (1A, 1B, 2A, 2B) and the variables: time and balloon volume was analyzed. In each series of the time variable, half of the animals had a 0.8mL balloon inflated for three minutes (Group 1A), and the other half had a 0.8mL balloon inflated for ten minutes (Group 2A). In the variable balloon volume series, half of the animals had a 0.6mL balloon inflated for 6 minutes (Group 1B), while the other half had a 0.8mL balloon inflated for 6minutes (Group 2B). In the qualitative and quantitative analysis of the parameters, the shortest group (group 1A), either there was no lesion, or there was partial loss of nervous tissue, while the longest group (group 2A) presented edema and cerebral parenchymal necrosis, reaching 35% of nuclear pyknosis. In the volume dependent groups (groups 1B and 2B), the findings were similar, both with about 30% of pyknotic nuclei. Thus, time was the major determinant of injury, reiterating the prognostic importance of early removal of a spontaneous intracerebral mass.


brainischemia, Caudate nucleus, Immunohistochemical, intracerebral mass, S100 proteins, World Health Organization, WHO, stroke, hemorrhage, Immunohistochemical, environment