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Blood transfusion and diagnostic accuracy of chagas disease


Journal of Bacteriology & Mycology: Open Access
Clever Gomes,1 Eduardo J Santos,1 Elisangela Silveira-Lacerda,1 Marcilio Baliza,2 Antonio R  Teixeira3

Abstract

Introduction: Disclaim of the Chagas disease case in Blood Center is based on the immunofluorescence and enzyme-linked immunosorbent antibody assays. 
Methods: The antibody assays were conducted in parallel with the nuclear DNA-PCR test in blood samples of 145 families’ members.  
Result: The specific antibody tests were positive in 26.9% (39/145) subjects. Contrastingly, the Trypanosoma cruzi nuclear DNA-PCR was positive in 72.4% (105/145) of those blood samples. 
Conclusion: The immune tolerance herein explains the reported discrepancies of results between the specific antibody and the accurate DNA analysis. The warranty of safe blood transfusion is achieved by the PCR technology.
Introduction: Disclaim of the Chagas disease case in Blood Center is based on the immunofluorescence and enzyme-linked immunosorbent antibody assays. 
Methods: The antibody assays were conducted in parallel with the nuclear DNA-PCR test in blood samples of 145 families’ members.  
Result: The specific antibody tests were positive in 26.9% (39/145) subjects. Contrastingly, the Trypanosoma cruzi nuclear DNA-PCR was positive in 72.4% (105/145) of those blood samples. 
Conclusion: The immune tolerance herein explains the reported discrepancies of results between the specific antibody and the accurate DNA analysis. The warranty of safe blood transfusion is achieved by the PCR technology.
 

Keywords

chagas Disease, immune tolerance, nuclear DNA-PCR, diagnosis accuracy, blood transfusion

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