Molecular characterization of a decapod Penstylidensovirus 1 (PstDV1) isolated from Penaeus semisulcatus in Egypt
- Journal of Aquaculture & Marine Biology
Mohamed E Megahed, Mohamed Fathi
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This study demonstrates the existence of decapod Penstylidensovirus 1 (PstDV1), in P.semisulcatus in Dibah Triangle Zone (DTZ), Egypt. The prevalence of infectious was studied in post larvae and juveniles at hatchery and farm levels. A survey of the PstDV1 prevalence in P. semisulcatus was undertaken in commercial shrimp hatcheries and farms, using polymerase chain reaction (PCR) to amplify a fragment of PstDV1, which encompasses the coding sequence for one of the viral coat proteins. The results showed that PstDV1 virus was responsible on high mortalities and deformity in the P. semisulcatus. The PCR screening of PstDV1 infection in shrimp hatcheries and farms allowed rejection of infected broodstock and postlarvae. The prevalence of PstDV-1 was high in all hatcheries and grow-out ponds surveyed, indicating that the virus was prevalent in aquaculture facilities at DTZ with statistically significant samples. This study recommends strict biosecurity measures are advisable for the continuity of P.semisulcatus culture in Egypt.
Penaeus semisulcatus, IHHNV, stunted growth, farm, hatchery, viral, bacterial, fungal, protozoan diseases, chemotherapeutics, mortalities