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Can connarus rubber extract inhibit the induction of insulin resistance by hyperglycemia?

MOJ Toxicology
Satomi Kawaguchi,1 Riki Kawasaki,1 Ryo Murashige,2 Kayoko Yamazaki,2 Shin ichiro Kurimoto,3 Takanori Nakamura,2 Yu F Sasaki1

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Although it would be ideal if the induction of insulin resistance by the persistence of the hyperglycemic state could be prevented by food components that can be consumed daily, to our knowledge, it has not been reported food components that have such effect. Advanced glycation end products (AGE) is involved in the development and progression of diabetic complications. Here, we investigated whether Connarus rubber (CR) extract can inhibit the production of AGE and the induction of insulin resistance. Twenty-four hours prior to the cultivation under serum-free condition, cells were exposed to CR extract and the glucose concentration in the medium was measured every 24 hours. Glucose intake was promoted in the presence of insulin without but not with hyperglycemia condition, showing that insulin resistance was induced in HepG2 cells under hyperglycemia condition. At 1.25 and 2.5µg/mL CR, glucose concentration decreased time-dependently with statistical significance, showing that the induction of insulin resistance was inhibited by CR extract. Furthermore, CR at >1μg/mL suppressed glycation of human serum albumin (HSA) and oxidation of glycated HSA in a concentration-dependent manner. In conclusion, CR is shown to inhibit the chemical reaction of AGE production and the induction of insulin resistance.


insulin resistance, glycated HAS, diabetic complications, HepG2 cells, glycated HAS, T2DM, pharmacological purposes, hypoglycemic, diabetic, type-2 diabetes mellitus, connaraceae,