Abstract

Surface sampling techniques for non-viable fungi in building environments are useful tools for investigators in determining hazards to occupants. However, data regarding capture efficiency in this context is limited. Our data demonstrates that collection efficiency of Bio-Tape surface capture medium on paper-faced gypsum board only captures between half and three-quarters of mould present on the surface. Surface sampling using a dry-swab technique showed similar efficiency of capture to tape lift samples. ‘Surface air’ samples had poor collection efficiency and should be avoided where possible in preference to other sampling options. Finally, we propose a sampling strategy based on non-viable microscopy techniques followed by molecular analysis for validation and speciation of samples of interest. Improvements in sampling and data analysis techniques for mould sampling of buildings will aid in providing meaningful results to help building inspectors evaluate health hazards.

Keywords

mould inspection, fungal sampling, non-viable, surface sampling, collection efficiency, capture efficiency, bio-tape, tape-lift, swab, fungi, building inspection, surface-air sampling