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Identification of a gene that makes a protein by using a cell free protein synthesis system


Abstract

A living cell could be genetically modified to perform a function such as the production of a protein. However, these genetic modifications often conflict with normal cellular function and result in a mutation. Defects can be overcome through removing the bacterial membrane which leaves the lysate that is performing both transcription and translation.

Keywords

protein synthesis, molecular size, polyacrylamide gels

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