Identification of a gene that makes a protein by using a cell free protein synthesis system
- Journal of Applied Biotechnology & Bioengineering
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Umair Masood
Abstract
A living cell could be genetically modified to perform a function such as the production of a protein. However, these genetic modifications often conflict with normal cellular function and result in a mutation. Defects can be overcome through removing the bacterial membrane which leaves the lysate that is performing both transcription and translation.
Keywords
protein synthesis, molecular size, polyacrylamide gels