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Ameloblastic Carcinoma: Clinico-Pathological and Immunohistochemical Claudin Study


Journal of Dental Health, Oral Disorders & Therapy
Gabriel Fernando Paredes-Ferrera1, Constantino Ledesma-Montes2, María Dolores Jiménez-Farfán2, Carlos Contreras-Castellanos2, José Espinoza-Fernández1, Florentino Hernández-Flores2, Alejandro Macario-Hernández2, Claudio Viveros-Amador2 and Juan Carlos Hernández-Guerrero2*
Department of Dentistry, National Autonomous University of Mexico, Mexico
Gabriel Fernando Paredes-Ferrera, Department of Oral Surgery, General Hospital of Mexico, Mexico
Constantino Ledesma-Montes, Department of Dentistry, National Autonomous University of Mexico, Mexico
Mar, Department of Dentistry, National Autonomous University of Mexico, Mexico
Carlos Contreras-Castellanos, Department of Dentistry, National Autonomous University of Mexico, Mexico
Jos, Department of Oral Surgery, General Hospital of Mexico, Mexico
Florentino Hern, Department of Dentistry, National Autonomous University of Mexico, Mexico
Alejandro Macario-Hern, Department of Dentistry, National Autonomous University of Mexico, Mexico
Claudio Viveros-Amador, Department of Dentistry, National Autonomous University of Mexico, Mexico

Abstract

1.1.    Background: Ameloblastic carcinoma (AC) is a rare and locally aggressive malignant odontogenic neoplasm found in the maxillomandibular area. Altered expression of claudins is associated to increased proliferation, loss of polarity and cell to cell adhesion, reorganization of the cytoskeleton and motility in cancer. Clinico-pathological characteristics and claudin immunoexpression was made in an AC derived from a unicystic ameloblastoma (UA).

1.2.    Methods: We studied an AC in a 18-year-old woman, performing immunostaining with anti-PCNA and claudin-1, -3 and -9 antibodies.

1.3.    Results: A malignant ill-differentiated neoplasm, composed by densely packed epithelial cells, and arranged in a solid growth pattern was found. Peripheral cells were ameloblastic, and central cells presented variable shapes with hyperchromatic, pleomorphic nuclei, prominent nucleoli and increased nucleus-cytoplasm ratio. Areas suggesting early neoplastic invasion were detected. The tumor was immunopositive to claudin-1 in cytoplasm and nucleus and negative to claudins -3 and -9.

1.4.    Conclusion: AC derived from a UA showed a heterogenic claudins immunostaining. The altered claudin immunoexpression in the tumor and early invasion areas, coinciding with PCNA positive proliferative cells, suggest their important role in tumorigenesis and support the theory that claudins are highly tissue-specific and their functions may depend on the cell type and the tumor stage.
 

Keywords

Odontogenic, Tumors, Ameloblastic, Carcinoma, Ameloblastoma, Unicystic, Ameloblastoma, Claudins, Tight junction, Proteins, Claudins

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