The Effects Induced Hyperglycemia on Adrenal Cortex Function in the Giant Danio Devario Aequipinnatus Embryos
- Journal of Diabetes, Metabolic Disorders & Control
-
Manickam Raja, Anbarasu Jayanthi, Mathiyalagan Kavitha and Pachiappan Perumal*
Department of Biotechnology, Periyar University, India - Manickam Raja, Department of Biotechnology, Periyar University, India
- Anbarasu Jayanthi, Department of Biotechnology, Periyar University, India
- Mathiyalagan Kavitha, Department of Biotechnology, Periyar University, India
Abstract
Purpose: The purpose of this study was to induce hyperglycemic experiment in Giant Danioembryos in relation to effects of hyperglycemia on adrenal cortex function and steroidogenesis.
Methods: The wild specimens of Devario aequipinnatusfishes collected from the Cauvery river at Stanley Reservoir were induced for breeding without the use of any hormones and the matured embryos were obtained. The embryos of Giant Daniowere induced for hyperglycemic stress, by immersing them in 0.0%, 0.3%, 0.6%, 0.9%, 1.2%, 1.5%, and 1.8% glucose solution, at 24 h, 48 h and 72 h.
Result: The Giant Danioexposed to glucose solution appear to experience acute stress as indicated by the significant elevation in serum glucose and serum cortisol. The ranges of average cortisol level for all embryo samples exposed for 24 h, 48 h and 72 h in stock aquaria were: 1.2-6.4 µg/dL, 0.8 to 7.6 µg/dL and 0.8-9.6µg/dL respectively.
Conclusion: The results showed positive correlation between the %glucose solution and the cortisol level, as well as the incubation time and the cortisol level as reported previously in other model systems.
Keywords
Diabetes Mellitus, Hyperglycemia, Cortisol, Giant Danio, Glucose, Serum, Incubation, Neuroendocrine, Biochemical, Adrenocorticotrophic, Corticotropin, Cyprinidae, , Blood, Pressure, Fish
