Anti-inflammatory effect of Ethanolic Extract of Bougainvillea x buttiana on LPS-induced J774A.1 macrophages
- Pharmacy & Pharmacology International Journal
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Mayra Cedillo Cortezano, Vera L Petricevich
Abstract
Introduction: Bougainvillea is used in Mexico to treat of various disorders. It is largely
used in folk medicine as an infusion to gastrointestinal and respiratory disorders to treat
cough, asthma, bronchitis and as laxative. To know the direct activity of this plant species
on immune system cells the present study evaluated the immunostimulant activities of
ethanolic extract from Bougainvillea x buttiana (Orange) (BxbOE) in murine macrophage
cell line J774A.1.
Methods: BxbOE extract was tested on J774A.1 cells to prove their immune-stimulant effect as determines by the viability, phagocytic index and mediators production. Methylthiazolydiphenyltetrazolium bromide (MTT) and Griess assays were performed to evaluate cell viability and nitric oxide (NO) production, respectively. The phagocytic activity was determined by non-opsonized zymosan was addition and the amount of engulfed zymosan. While the cytokines levels were tested by enzyme-linked immunosorbent assay (ELISA) method.
Results: Concentrations of BxbOE at 200 to 400 μg/mL had cytotoxicity on J774A.1 cells LPS- stimulated, however, while in cells treated with lower concentrations (from 0.01 to 100 μg/mL) did not produce significant cytotoxic effects and not exhibited an inhibitory effect phagocytic index. Our results showed that the BxbOE can inhibit the pro-inflammatory mediators production including nitric oxide and cytokines comprising IL- 1β, TNF-α and IL-6, and an increment of IL-10 levels.
Conclusion: Our results help to aggregate for literature data on the immunostimulation activity of this plant species, cell from the immune system involved in the innate and acquired immune response, the macrophages.
Methods: BxbOE extract was tested on J774A.1 cells to prove their immune-stimulant effect as determines by the viability, phagocytic index and mediators production. Methylthiazolydiphenyltetrazolium bromide (MTT) and Griess assays were performed to evaluate cell viability and nitric oxide (NO) production, respectively. The phagocytic activity was determined by non-opsonized zymosan was addition and the amount of engulfed zymosan. While the cytokines levels were tested by enzyme-linked immunosorbent assay (ELISA) method.
Results: Concentrations of BxbOE at 200 to 400 μg/mL had cytotoxicity on J774A.1 cells LPS- stimulated, however, while in cells treated with lower concentrations (from 0.01 to 100 μg/mL) did not produce significant cytotoxic effects and not exhibited an inhibitory effect phagocytic index. Our results showed that the BxbOE can inhibit the pro-inflammatory mediators production including nitric oxide and cytokines comprising IL- 1β, TNF-α and IL-6, and an increment of IL-10 levels.
Conclusion: Our results help to aggregate for literature data on the immunostimulation activity of this plant species, cell from the immune system involved in the innate and acquired immune response, the macrophages.
Keywords
Bougainvillea x buttiana, nitric oxide, cytokines, phagocytosis, antiinflammatory
