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Molecular characterisation of pathogens isolated from egg samples

International Journal of Molecular Biology: Open Access
P Sagadevan,1 Jenifer Christina,1 M Raghunath,1 M Nagarajan,1 S Premalatha,1 R Preeethi,1 Janarthanan P2


ceA on the biochemical analysis of this study, which is similar to a battery of biochemical
tests including methyl red, Voges-Proskauer, indole and citrate, the organisms were
determined to be Klebsiella. In the present study, the highest resistance rate was observed
for inoflavuxin (50%) and ceftazidimine (80%), which is similar to the highest resistance
rate for cefotaxime (85%) and ceftazidime (68%), with the MIC ranging from 32 to 256
mg/l. The Pseudomonas and Klebsiella plasmid isolation in the present study was found to
have a molecular weight of 2.6 kb and 2.7 kb, which is similar to most isolates harboring
multiple plasmids ranging in size from about 2 kb to over 150 kb included. Seventy-nine
isolates contained plasmids larger than 50 kb, and some contained multiple large plasmids.
In the present study, the molecular weight of Pseudomonas and Klebsiella was found to be
356 base pairs and 359 base pairs, respectively, which is similar to that of the 85 clinical
isolates of Klebsiella pneumonia screened by PCR amplification of the antiseptic resistance
genes qacE1 and ceA.


pseudomonas, klebsiella, pathogens, molecular characterization