This research endeavors to elucidate the potential association between urinary microbiota and the prevalence of female pelvic organ prolapse (POP). Leveraging state-of-art metagenomics analysis, we scrutinize the microbial landscape within the urinary tract of individuals with POP, justaposed against a cohort study and summarizing the results of all the correlated papers that approach this subject. Through meticulous study and research, it is aimed to pinpoint distinctive and diverse microbial patterns related with POP development. Findings of the review aspire to shed light on the intricate dynamics between urinary microbiota and female pelvic health, offering nuanced insight into the contributory factors underpinning pelvic organ prolapse. This research not only deepens the understanding of the microbial aspect of POP but also holds promise for informing potential diagnostic and therapeutic avenues in the realm of female pelvic floor disorders.