Tsetse flies (Glossina spp.) are vectors of African trypanosomiasis, a disease of significant concern in sub-Saharan Africa. An understanding of the dynamics of tsetse fly populations is crucial for effective disease management. This study investigates the population dynamics of tsetse flies in the Ido Local Government Area (LGA) of Oyo State, Nigeria, and their response to environmental factors and climate change. Through field observations and data analysis, correlations between temperature, humidity, vegetation cover, and tsetse fly presence were identified. Results reveal positive associations between temperature and tsetse fly presence, highlighting the importance of warmer temperatures for fly activity. Additionally, strong positive correlations with vegetation cover suggest the significance of suitable habitats for fly survival. The role of humidity in shaping fly habitats warrants further investigation. These findings provide insights into the environmental determinants of tsetse fly ecology in the Ido LGA, with implications for disease transmission dynamics. Targeted interventions informed by this study can aid in controlling tsetse-borne diseases and protecting human and animal health in the region.