This study aims to construct a gene map of Qiang embroidery motifs based on cultural gene theory, through the structural analysis of both dominnant and recessive factors. The approach involves organizing six elements—motif themes, forms, colors, composition, craftsmanship, and semantics—to build a gene map that respects the cultural context of the Qiang people, and then inductively extract the fundamental elements for innovative pattern design. The construction of a cultural gene map for Qiang embroidery motifs provides a more comprehensive and holistic reflection of the cultural characteristics and aesthetic meanings of the Qiang people. The design translation of cultural genes contributes to the effective expression and inheritance of ethnic culture, enhances consumers' cultural identity, and serves as a bridge connecting culture with design creation. It also offers an accedemic reference for the innovation of ethnic cultural and creative products.