Abstract

Plant based amylases are affordable and sustainable alternative which are exploited these days to get immobilized by adsorption, entrapment, covalent attachment, and cross-linking. Amylases is well known thermostable enzyme (stable at 80-90°C) to economize various industrial processes like, leather, paper, wood, detergent, refinery, distillery and starch industries. Thermostable enzymes isolated from thermophilic organisms have found several commercial applications because of their overall inherent stability. Improved, cost effective and green method of enzyme immobilization was developed to immobilize Vigna mungo amylase onto activated cotton fabric with ease. 80% Retention of immobilized Vigna mungo Amylase was achieved onto woven activated cotton fabric (80%) when compared to inactivated woven cotton fabric (15%). Various Kinetic parameters were studied for free and immobilized enzyme for finding its improved stability on varying pH, time of incubation, temperature, CaCl2 Concentration. So, that immobilized amylase would further propose for various industrial applications like food, chemical, textiles and paper industries and used for various chemical/biological conversions for synthesising novel materials when found better for its improved kinetic parameters with free amylase. 

Keywords

amylase, immobilization, Vigna mungo amylase, cotton fabric